Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome
Identifieur interne : 004C36 ( Main/Exploration ); précédent : 004C35; suivant : 004C37Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome
Auteurs : David Bramhill [États-Unis] ; Arthur Kornberg [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1988.
English descriptors
- Teeft :
- Acad, Amppcp, Assay, Bglll, Bglll sites, Biol, Chromosome, Cleavage, Cleavage sites, Coli, Complex formation, Datp, Deletion, Densitometric scanning, Dna, Dnaa, Dnaa boxes, Dnaa protein, Dnab, Dnab helicase, Dnac, Dnac proteins, Duplex, Duplex opening, Edta, Ende, Endonuclease, Enzymatic replication, Escbericbia, Escbericbia chromosome, Escherichia, Ethidium bromide, Experimental procedures, Fmol, Funnell, Helicase, High temperature, Hindlll, Kornberg, Leftmost, Linear molecules, Linearization, Messer, Mutant, Mutation, Mutational changes, Natl, Nuclease, Nuclease sensitivity, Nucleotide, Nucleotide requirements, Nucleotide sequence, Ogawa, Open complexes, Oric, Oric sequences, Plasmid, Polyacrylamide denaturing, Polymerase, Polynucleotide kinase, Prepriming, Prepriming complexes, Primase, Proc, Promoter, Protein, Reconstituted, Replication, Replication origin, Rightmost, Sekimizu, Stable prepriming, Supercoiled, Tandem, Temperature profiles, Unpublished data, Upper strand, Zyskind.
Abstract
Abstract: Three tandem repeats of a 13-mer in the AT-rich region are essential to the unique replication origin of E. coli and of remotely related Enterobacteriaceae. These iterated sequences are identified by deletion analysis and sensitivities to endonucleases as the site for initial duplex opening by the initiator dnaA protein. This “open complex” requires ATP and 38°C for optimum formation and stability. The subsequent dnaC-dependent entry of dnaB helicase to form a “prepriming complex” stabilizes the open structure, blocks cleavages by a restriction endonuclease in the 13-mer region, and broadens the endonuclease cutting pattern. We propose that dnaA protein recognizes and successively open the 13-mer sequences, thereby guiding the entry of dnaB helicase into the duplex preparatory to priming of replication.
Url:
DOI: 10.1016/0092-8674(88)90412-6
Affiliations:
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temperature</term><term>Hindlll</term><term>Kornberg</term><term>Leftmost</term><term>Linear molecules</term><term>Linearization</term><term>Messer</term><term>Mutant</term><term>Mutation</term><term>Mutational changes</term><term>Natl</term><term>Nuclease</term><term>Nuclease sensitivity</term><term>Nucleotide</term><term>Nucleotide requirements</term><term>Nucleotide sequence</term><term>Ogawa</term><term>Open complexes</term><term>Oric</term><term>Oric sequences</term><term>Plasmid</term><term>Polyacrylamide denaturing</term><term>Polymerase</term><term>Polynucleotide kinase</term><term>Prepriming</term><term>Prepriming complexes</term><term>Primase</term><term>Proc</term><term>Promoter</term><term>Protein</term><term>Reconstituted</term><term>Replication</term><term>Replication origin</term><term>Rightmost</term><term>Sekimizu</term><term>Stable prepriming</term><term>Supercoiled</term><term>Tandem</term><term>Temperature profiles</term><term>Unpublished data</term><term>Upper strand</term><term>Zyskind</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Three tandem repeats of a 13-mer in the AT-rich region are essential to the unique replication origin of E. coli and of remotely related Enterobacteriaceae. These iterated sequences are identified by deletion analysis and sensitivities to endonucleases as the site for initial duplex opening by the initiator dnaA protein. This “open complex” requires ATP and 38°C for optimum formation and stability. The subsequent dnaC-dependent entry of dnaB helicase to form a “prepriming complex” stabilizes the open structure, blocks cleavages by a restriction endonuclease in the 13-mer region, and broadens the endonuclease cutting pattern. We propose that dnaA protein recognizes and successively open the 13-mer sequences, thereby guiding the entry of dnaB helicase into the duplex preparatory to priming of replication.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Bramhill, David" sort="Bramhill, David" uniqKey="Bramhill D" first="David" last="Bramhill">David Bramhill</name></noRegion><name sortKey="Kornberg, Arthur" sort="Kornberg, Arthur" uniqKey="Kornberg A" first="Arthur" last="Kornberg">Arthur Kornberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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